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FRISC: The Faculty Research Interests Science Comparator |
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Assistant Professor of Dermatology
Immunology
Office: 214-648-7552
FAX: 214-648-9292
Email: kariiz@mednet.swmed.edu
A pathway of costimulation that prevents anergy in CD28- T cells: B7-independent costimulation of CD1-restricted T cells.
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Score: 0.398 |
Dendritic cells (DC) are the most potent initiators of primary
antigen-specific immune responses among antigen presenting cells (APC), including B cells
and macrophages. Our ultimate goal is to characterize the molecular mechanisms by which DC
express that potency. As a first step, we have identified recently two genes, dectin-1 and
dectin-2, by a subtractive cDNA cloning method in which a cDNA library, prepared from a DC
line, was subtracted with mRNA isolated from the J774 macrophage line. We have determined
that dectin-1 and dectin-2 are both expressed by DC, but not by B cells, macrophages, or
other cell lines. With respect to function, we found that both molecules are required for
DC-mediated T cell activation. Thus, we have concluded dectin-1 and dectin-2 are members
of the costimulatory family. We have also observed that dectin-1 interacts with a
polypeptide(s) (dectin-1 ligand) that is (are) expressed on activated T cells.
Modulation of the interactions between costimulatory molecules and their
respective ligands is now utilized experimentally to regulate certain immune reactions,
including transplantation reactions (induction of anergy or tolerance) and tumor
recognition. Thus, biological reagents that interrupt the interaction of costimulatory
molecules/ligands are powerful tools for regulation of immune responses. We have proposed
that blockage of Dec1/ligand interaction may be applicable for these purposes. In summary,
we plan to identify and characterize ligands for dectin-1 and dectin-2. Our studies should
provide important insight into molecular mechanisms of DC-mediated T cell activation.
Moreover, we believe that the knowledge and reagents developed in these studies will be
applicable to the development of new strategies in regulating the to regulate the
magnitude, type, and direction of immune responses during tumor immunotherapy and in other
clinical settings.
Selected Publications:
Ariizumi K, Meng Y, Bergstresser PR, Takashima A (1995) IFN-(-dependent
IL-7 gene regulation in keratinocytes. J Immunol 154:6031-6039
Takashima A, Edelbaum D, Kitajima T, Shadduck RK, Gilmore G, Xu S,
Bergstresser PR, Ariizumi K (1995) Colony-stimulating factor-1 secreted by fibroblasts
promotes the growth of dendritic cell lines (XS series) derived from murine epidermis. J
Immunol 154:5128-5135
Ariizumi K, Kitajima T, Bergstresser PR, Takashima A (1995) IL-1beta
converting enzyme in murine Langerhans cells and epidermal-derived dendritic cell lines. Eur
J Immunol 25:2137-2140
Takashima A, Matsue H, Bergstresser PR, and Ariizumi K (1995)
IL-7-dependent interaction of dendritic epidermal T cells with keratinocytes. J Invest
Dermatol 105:50s-53s
Ariizumi, K, Xu S, PR Bergstresser, A Takashima (1995)
Subtractive cloning of genes expressed preferentially by dendritic cells (DC). J Invest
Dermatol 104
Ariizumi K, Shen G-L, Ritter R, Edelbaum D, Bergstresser PR and
Takashima A (1997) Identification of Dectin-2, a second member of a new dendritic
cell-associated C-type lectin family. J Invest Dermatol 108:564
Page maintained by Stephanie Robertson
Last Updated: 17 Nov 2000
Extraction Method: Expand using Medical Synonyms-
Eliminated words list: MedlinePlus List
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Similarity Method: Weighted keyword count
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Database: Medline abstracts (1967 - Present)
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Publication Type: All
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Score Calculation Method: Cosine Similarity Method
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Results computed on: 6/9/2006