RIC > Researcher Profiles > McEwan, William

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RIC: Research Interest Comparator

McEwan, William, Ph.D.
Affiliation: not provided
Email: not provided
Home Page: not provided
All Results | New This Month | Abstract | Selected Publications | RIC Statistics Results - NEW THIS YEAR:

	The human TRIM5alpha restriction factor mediates accelerated uncoating of the N-tropic murine leukemia virus capsid. Michel J Perron ... Joseph Sodroski J Virol 2007 Mar; 81(5)2138-48.	Score: 0.881
	The presence of the Trim5alpha escape mutation H87Q in the capsid of late stage HIV-1 variants is preceded by a prolonged asymptomatic infection phase. Neeltje A Kootstra ... Hanneke Schuitemaker AIDS 2007 Oct; 21(15)2015-23.	Score: 0.782
	Inhibition of HIV-1 replication by simian restriction factors, TRIM5alpha and APOBEC3G. R Sakuma ... Y Ikeda Gene Ther 2007 Jan; 14(2)185-9.	Score: 0.733
	Unique features of TRIM5alpha among closely related human TRIM family members. Xing Li ... Joseph Sodroski Virology 2007 Apr; 360(2)419-33.	Score: 0.713
	Restriction of an extinct retrovirus by the human TRIM5alpha antiviral protein. Shari M Kaiser ... Michael Emerman Science 2007 Jun; 316(5832)1756-8.	Score: 0.693
	The control of viral infection by tripartite motif proteins and cyclophilin A. Greg J Towers Retrovirology 2007 ; 4()40.	Score: 0.688
	A brief history of TRIM5alpha. Ruchi M Newman ... Welkin E Johnson AIDS Rev 2007 Apr-Jun; 9(2)114-25.	Score: 0.652
	Functional interplay between the B-box 2 and the B30.2(SPRY) domains of TRIM5alpha. Xing Li ... Joseph Sodroski Virology 2007 Sep; 366(2)234-44.	Score: 0.650
	Modulation of Retroviral Restriction and Proteasome Inhibitor-Resistant Turnover by Changes in the TRIM5{alpha} B-Box 2 Domain. Felipe Diaz-Griffero ... Joseph Sodroski J Virol 2007 Oct; 81(19)10362-78.	Score: 0.640
	Wild type and H43Y variant of human TRIM5alpha show similar anti-human immunodeficiency virus type 1 activity both in vivo and in vitro. Emi E Nakayama ... Ioannis Theodorou Immunogenetics 2007 Jun; 59(6)511-5.	Score: 0.622
	The design of artificial retroviral restriction factors. Melvyn W Yap ... Jonathan P Stoye Virology 2007 Sep; 365(2)302-14.	Score: 0.620
	Rhesus monkey TRIM5alpha restricts HIV-1 production through rapid degradation of viral Gag polyproteins. Ryuta Sakuma ... Yasuhiro Ikeda Nat Med 2007 May; 13(5)631-5.	Score: 0.601
	An active TRIM5 protein in rabbits indicates a common antiviral ancestor for mammalian TRIM5 proteins. Torsten Schaller ... Greg J Towers J Virol 2007 Nov; 81(21)11713-21.	Score: 0.594
	Alpha Interferon Enhances TRIM5{alpha}-Mediated Antiviral Activities in Human and Rhesus Monkey Cells. Ryuta Sakuma ... Yasuhiro Ikeda J Virol 2007 Sep; 81(18)10201-6.	Score: 0.554
	A Single Amino Acid of the Human Immunodeficiency Virus Type 2 Capsid Affects Its Replication in the Presence of Cynomolgus Monkey and Human TRIM5{alpha}s. Haihan Song ... Tatsuo Shioda J Virol 2007 Jul; 81(13)7280-5.	Score: 0.551
	Fusion of cyclophilin a to fv1 enables cyclosporine-sensitive restriction of human and feline immunodeficiency viruses. Torsten Schaller ... Greg J Towers J Virol 2007 Sep; 81(18)10055-63.	Score: 0.516
	The Retroviral Restriction Factor TRIM5alpha. Sarah Sebastian ... Jeremy Luban Curr Infect Dis Rep 2007 Mar; 9(2)167-73.	Score: 0.506
	A TRIM5alpha-independent post-entry restriction to HIV-1 infection of macaque cells that is dependent on the path of entry. Mario Javier Pineda ... Julie Overbaugh Virology 2007 Jul; 363(2)310-8.	Score: 0.501

Abstract:
The antiretroviral protein TRIM5alpha is known to have evolved different restriction capacities against various retroviruses, driven by positive Darwinian selection. However, how these different specificities have evolved in the primate lineages is not fully understood. Here we used ancestral protein resurrection to estimate the evolution of antiviral restriction specificities of TRIM5alpha on the primate lineage leading to humans. We used TRIM5alpha coding sequences from 24 primates for the reconstruction of ancestral TRIM5alpha sequences using maximum likelihood and Bayesian approaches. Ancestral sequences were transduced into HeLa and CRFK cells. Stable cell lines were generated and used to test restriction of a panel of extant retroviruses (HIV-1, HIV-2, SIVmac, SIVagm, N-MLV and B-MLV). The resurrected TRIM5alpha variant from the common ancestor of Old World primates (Old World monkeys and apes, approximately 25 million years before present) was effective against modern/current day HIV-1. Contrary to the HIV-1 restriction pattern, we show that restriction efficacy against other retroviruses, such as a murine oncoretrovirus (N-MLV), is higher for more recent resurrected hominoid variants. Ancestral TRIM5alpha variants have generally limited efficacy against HIV-2, SIVagm, and SIVmac.. Our study sheds new light on the evolution of the intrinsic antiviral defense machinery and illustrates the utility of functional evolutionary reconstruction for characterizing recently emerged protein differences.

Keywords extracted from the abstract: [ eliminated words list ]

Count	Word
3.247	ancestor
9.930	ancestral
2.616	antiretroviral
4.314	antiviral
3.718	apes
1.964	approaches
1.454	approximately
6.000	b-mlv
3.145	bayesian
2.915	capacities
0.664	cell
0.616	cells
2.900	characterizing
2.118	coding
1.376	common
2.472	contrary
4.548	crfk
1.462	current
4.110	darwinian
1.280	day
2.348	defense
1.256	differences
2.050	different
2.624	driven
1.396	effective
3.259	efficacy
2.575	emerged
2.105	estimate
3.676	evolution
2.386	evolutionary

Count	Word
5.369	evolved
3.448	extant
2.043	fully
1.367	functional
1.884	generally
1.856	generated
2.187	hela
1.114	higher
5.222	hiv-1
6.227	hiv-2
4.185	hominoid
1.848	humans
2.836	illustrates
2.191	intrinsic
1.431	known
1.912	leading
1.529	light
2.400	likelihood
1.723	limited
2.476	lineage
2.783	lineages
1.587	lines
2.761	machinery
1.701	maximum
2.356	million
2.138	modern
2.223	monkeys
0.902	more
1.819	murine
12.198	n-mlv

Count	Word
3.614	old
5.455	oncoretrovirus
0.926	other
2.378	panel
1.475	pattern
1.295	positive
5.384	primate
5.337	primates
1.910	protein
1.555	recent
1.689	recently
4.161	reconstruction
6.479	restriction
10.004	resurrected
4.430	resurrection
8.035	retroviruses
1.731	selection
4.181	sequences
3.953	sheds
8.878	sivagm
8.305	sivmac
5.503	specificities
1.734	stable
2.916	transduced
23.500	trim5alpha
2.259	understood
2.578	used
0.886	using
2.333	utility
2.078	variant

Count	Word
4.302	variants
1.344	various
3.824	world
1.053	years

RIC Statistics:

Extraction Method: Keyword Count with Lexical Variants Added

Eliminated words list: MedlinePlus List

Weighting Method: Term Frequency * Inverse Document Frequency

Database: Medline Updates from current year

Publication Type: All

Score Calculation Method: Cosine Similarity Method

Sort by: Score

Submission date and time: 1-30-2008, 10:27:3

Computation time: 00:00:13

Last updated: Wednesday, 30-Jan-2008 10:27:16 CST